Miniaturisation and optimisation of the Dutch mini-Luke extraction method for implementation in the routine multi-residue analysis of pesticides in fruits and vegetables

作者：Lozano Ana^*; Kiedrowska Barbara; Scholten Jos; de Kroon Marijke; de Kok Andre; Fernandez Alba Amadeo R

来源：Food Chemistry, 2016, 192: 668-681.

DOI：10.1016/j.foodchem.2015.07.065

• New Dutch mini-Luke (NL-) method
• Miniaturisation
• Liquid chromatography
• Gas chromatography
• Mass spectrometry
• Validation

This paper presents the validation and further miniaturisation of the well-known Dutch mini-Luke method for high water and acid content matrices for 175 pesticides amenable to liquid- and gas chromatography-mass spectrometry. For optimisation of the method, recovery tests with different sample/extraction solvent ratios, varying amounts of dichloromethane and salts were performed with fifty representative pesticides. Solvent consumption could be reduced considerably, especially for the dichloromethane (by a factor of 3). Recovery studies performed with lettuce and orange matrix spiked at 0.005, 0.01 and 0.02 mg/kg yielded average recoveries in the range 70-120% with relative standard deviation values below 20% for almost all the pesticides tested. The linearity over three orders of magnitude was demonstrated (r(2)>0.99). The matrix effect could be considered as not significant. The limit of quantification was 0.005 mg/kg for 96% of the compounds. The optimised New Dutch mini-Luke ("NU-) method was applied successfully in routine analysis and the EUPT FV-16 sample.

• 出版日期2016-2-1