Nucleotides modify growth of selected intestinal bacteria in vitro

作者：Sauer N; Bauer E; Vahjen W; Zentek J; Mosenthin R^*

来源：Livestock Science, 2010, 133(1-3): 161-163.

DOI：10.1016/j.livsci.2010.06.053

• Nucleotide
• Intestinal bacteria
• Growth response
• In vitro

Dietary nucleotides play an important role in the development of the gastrointestinal tract and may affect intestinal bacteria in young monogastrics. Two in vitro studies have been performed to evaluate the effects of individually added nucleotides (AMP, CMP. GMP, IMP, and LIMP) at different concentrations on growth response of selected bacteria strains that dominate the piglets' small intestine. In experiment 1. a basal medium based on NH4Cl was used to evaluate the growth of three different Escherichia coli strains (DSM 2840, PS 37, and PS 79) using different nucleotide concentrations. In experiment 2, growth of Lactobacillus reuteri (DSM 20016), Lactobacillus amylovorus (DSM 20513), Enterococcus faecium (DSM 2146) and Enterococcus faecalis (DSM 6134) was assessed using two different media (based on yeast extract or casein) containing graduated levels of the respective nucleotides. In experiment 1, in vitro growth of the pathogenic E. coli strain PS 37 was more negatively affected by nucleotides when compared to the nucleotide free control medium, while the non-pathogenic E. coli DSM 2840 and the pathogenic strain PS79 showed an enhanced growth response compared to the control medium. In experiment 2, all tested strains were able to utilize the yeast extract medium more efficiently when compared to the casein medium, but no differences in the efficiency of added single nucleotides at any given concentration could be detected. These preliminary results suggest a potential role of nucleotides in affecting growth of intestinal bacteria.

• 出版日期2010-9