Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning

作者：Cheng Jun; Tiyaboonchai Amita; Yamashita Yukiko M^*; Hunt Alan J

来源：Development, 2011, 138(5): 831-837.

DOI：10.1242/dev.057901

• Stem cells
• Asymmetric division
• Spindle repositioning
• Drosophila

Many stem cells divide asymmetrically to balance self-renewal and differentiation. In Drosophila testes, two stem cell populations, germline stem cells (GSCs) and somatic cyst stem cells (CySCs), cohere and regulate one another. Here, we report that CySCs divide asymmetrically through repositioning the mitotic spindle around anaphase. CySC spindle repositioning requires functional centrosomes, Dynein and the actin-membrane linker Moesin. Anaphase spindle repositioning is required to achieve high-fidelity asymmetric divisions in CySCs, thus maintaining both GSC and CySC numbers. We propose that dynamic spindle repositioning allows CySCs to divide asymmetrically while accommodating the structure of the GSCs they encapsulate.

• 出版日期2011-3-1