Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains

作者：Song Keum Soo; Nimse Satish Ba****heb; Kim Hee Jin; Yang Jeongseong; Kim Taisun^*

来源：Sensors, 2016, 16(3): 376.

DOI：10.3390/s16030376

• sensitivity
• accuracy
• drug-resistant tuberculosis
• tuberculosis
• specificity
• diagnosis

In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%).

• 出版日期2016-3
• 单位中国科学院长春应用化学研究所