Usefulness of Immunoglobulin Light-Chain Restriction on Immunocytochemical Double Staining for the Cytological Diagnosis of B Cell Non-Hodgkin's Lymphoma

作者：Shimoura Yasumasa; Sato Yasuharu^*; Takata Katsuyoshi; Orita Yorihisa; Nakamura Satoko; Mano Shyouhei; Yoshino Tadashi

来源：Acta Cytologica, 2013, 57(1): 84-90.

DOI：10.1159/000342475

• Cytology
• Immunocytochemical double staining
• Immunoglobulin light-chain restriction
• Lymph node
• B cell lymphoma
• Flow cytometry

Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.

• 出版日期2013