A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the NF-kappa B Driven Transcription in Human Gastric Epithelial Cells

作者：Colombo Elisa; Sangiovanni Enrico; D'Ambrosio Michele; Bosisio Enrica; Ciocarlan Alexandru; Fumagalli Marco; Guerriero Antonio; Harghel Petru; Dell'Agli Mario^*

来源：Evidence-Based Complementary and Alternative Medicine, 2015, 2015: 727342.

DOI：10.1155/2015/727342

Calendula officinalis L. has been largely known for its topical anti-inflammatory properties; however, there are no experimental evidences about its antiphlogistic effect at the gastric level. To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2 extract obtained from C. officinalis flowers was evaluated in vitro on the NF-kappa B pathway. The lipophilic extract demonstrated a significant inhibitory effect on the NF-kappa B driven transcription. The identification of active compounds was conducted by a bio-guided fractionation of the extract that afforded 16 fractions. Fraction J exhibited a concentration-dependent inhibitory activity on the NF-kappa B driven transcription and significantly contributed to the antiphlogistic effect showed by CH2Cl2 extract. The main components of fraction J were loliolide and the fucoside acetates of beta-eudesmol and viridiflorol. HPLC analysis of fractions D and E led to the identification and isolation of triterpene esters that showed a concentration-dependent inhibition of the NF-kappa B driven transcription, with faradiol-3-myristate and the corresponding aglycone being the most active compounds. The present study provides some experimental evidences that Calendula officinalis L. may exert an anti-inflammatory activity on the gastric district by the inhibition of the NF-kappa B system, identifying the compounds responsible, at least in part, for the observed effect.

• 出版日期2015