科研之友
微信
新浪微博
Facebook
分享链接
摘要
Saikosaponin-d (Ssd), one of the major components extracted from Bupleurum falcatum L exhibits a variety of pharmaceutical activities including anti-tumor, anti-inflammation and immunomodulatory properties. In the current study, we investigated the protective effects of Ssd on MPP+-induced cytotoxicity in SH-SY5Y cells along with the underlying mechanisms. Our study showed that Ssd exerted a concentration-dependent neuroprotective effect against MPP+-induced reduction of cell viability in the in vitro model of Parkinson's disease. In addition, MPP+ exposure led to an increased oxidative stress, apoptotic cell deaths and caspase-3 activity, which was attenuated by incubation with Ssd. On the molecular level, we found that Ssd treatment significantly increased the mRNA and protein expression of SIRT3. Meanwhile, SIRT3 knockdown abolished the protective effects of Ssd on SH-SY5Y cells. Taken together, these results demonstrated that treatment of SH-SY5Y cells with Ssd could protect against MPP+-induced cytotoxicity via regulation of SIRT3 expression.
