Deoxynivalenol-induced cytokines and related genes in concanavalin A-stimulated primary chicken splenic lymphocytes

作者：Ren, Zhihua; Wang, Yachao; Deng, Huidan; Deng, Youtian; Deng, Junliang^*; Zuo, Zhicai; Wang, Ya; Peng, Xi; Cui, Hengmin; Shen, Liuhong; Ma, Xiaoping; Fang, Jing

来源：Toxicology in Vitro, 2015, 29(3): 558-563.

DOI：10.1016/j.tiv.2014.12.006

• Deoxynivalenol
• Mycotoxin
• Immunity
• Chicken
• Cytokines

Deoxynivalenol (DON) immunotoxicity and its induction of cytoldnes and related genes in the splenic lymphocytes of chickens have not been completely elucidated. In the present study, we aimed to evaluate the effects of 48 h of different DON treatments (0 mu g/mL, 0.2 mu g/mL, 0.8 mu g/mL, 3.2 mu g/mL, 12.5 mu g/mL, and 50 mu g/mL) on the secretion and the mRNA expressions of some cytokine genes, such as interleukin-1beta (IL-1 beta), IL-1RI, IL-2, IL-4, IL-6, IL-10, IL-12 beta, and IFN-gamma in chicken splenic lymphocytes. The concentrations of IL-1RI, IL-6, IL-10, and IFN-gamma were increased with the DON concentrations increasing (P < 0.05 or P < 0.01). However, the concentrations of IL-1 beta, IL-2, IL-4, and IL-12 beta were decreased with the DON concentrations increasing (P < 0.05 or P <0.01). Except IL-1 beta, the mRNA expressions of the other cytokines were up-regulated by DON. The highest mRNA expressions values of IL-1RI, IL-4, IL-10, IL-12 beta, and IFN-gamma were at 50 mu g/mL DON treatment groups (P < 0.05 or P < 0.01), while the highest mRNA expressions values of IL-2 and IL-6 were at 12.5 mu g/mL DON treatment groups (P <0.05 or P <0.01). Our data revealed that the potent effects of DON in affecting the secretion and the mRNA expression of the related cytokines in chicken splenic lymphocytes in vitro.

• 出版日期2015-4
• 单位四川农业大学; 西南科技大学