Deep imaging in scattering media with selective plane illumination microscopy

作者：Pediredla Adithya Kumar; Zhang Shizheng; Avants Ben; Ye Fan; Nagayama Shin; Chen Ziying; Kemere Caleb; Robinson Jacob T^*; Veeraraghavan Ashok^*

来源：Journal of Biomedical Optics, 2016, 21(12): 126009.

DOI：10.1117/1.JBO.21.12.126009

• microscopy
• scattering
• imaging
• selective plane of illumination microscopy

In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has emerged as the most common method to image deep within scattering biological tissue. This technique, however, requires high-power pulsed lasers that are both expensive and difficult to integrate into compact portable systems. Using a combination of theoretical and experimental techniques, we show that if the excitation path length can be minimized, selective plane illumination microscopy (SPIM) can image nearly as deep as 2PM without the need for a high-powered pulsed laser. Compared to other single-photon imaging techniques like epifluorescence and confocal microscopy, SPIM can image more than twice as deep in scattering media (similar to 10 times the mean scattering length). These results suggest that SPIM has the potential to provide deep imaging in scattering media in situations in which 2PM systems would be too large or costly.

• 出版日期2016-12