Decursin inhibits growth of human bladder and colon cancer cells via apoptosis, G1-phase cell cycle arrest and extracellular signal-regulated kinase activation

作者：Kim Wun Jae; Lee Se Jung; Choi Young Deuk; Moon Sung Kwon^*

来源：International Journal of Molecular Medicine, 2010, 25(4): 635-641.

DOI：10.3892/ijmm_00000386

• decursin
• bladder cancer cells
• colon cancer cells
• G1-phase cell cycle arrest
• apoptosis
• p21WAF1
• extracellular signal-regulated kinases

Decursin, a pyronocoumarin isolated from the Korean Angelica gigas root. has demonstrated anti-cancer properties In the present study, we found that decurisn inhibited cell variability In cutured humab Urinary bladder cancer 235J cells and colon cancer HCT116 cells The inhibited proliferation was due to apoptotic induction. because both cells treated With decursin dose-dependently showed a SUb-G1 phase accumulation and an increased cytoplasmic DNA-historic complex. Cell death caused by decursin was also associated with the down-regulation of pro-apoptotic factoer Bcl-2 and the up-regulation of pro-apoptotic molecules cytochrome c, caspase 3 and Bax Treatment of both types of cancer cells with decursin resulted in G1-phase cell cycle arrest, as revealed by FACS analyses In addition, decursin Increased protein levels of p21WAF1 with a decrease in cyclins and cychn dependent knases; (CDKs) Furthermore, decursin induced the activation of extracellular signal-regulated kinases (ERK) in both cancer cell lines. with the notable exceptions of c-Jun N-terminal kinase (JNK) and p38 mitogen activated protein (MAP) kinase Finally, pretreatment with ERK-specific Inhibitor PD98059 reversed decursin-induced p21WAFI expression and decursin-inhibited cell growth. Thus. these findings suggest that decursin has potential therapeutic efficacy for the treatment of bladder and colon cancer

• 出版日期2010-4