Anti-oxidative and anti-apoptotic roles of spermatogonial stem cells in reversing cisplatin-induced testicular toxicity

作者：Hussein Yousri M; Mohammed Randa H^*; Shalaby Sally M; Abd El Haleem M**** R; Abd El Motteleb Dalia M

来源：Cytotherapy, 2015, 17(11): 1646-1654.

DOI：10.1016/j.jcyt.2015.07.001

• anti-apoptotic
• anti-oxidant
• cisplatin
• infertility
• spermatogonial stem cell

Background aims. Because of reproductive toxic effects of chemotherapy, researchers have taken some techniques to preserve fertility potential. The present study was designed to point out the potential role of spermatogonial stem cell (SSC) therapy in reversing cisplatin (CP)-induced testicular toxicity and restore the spermatogenesis. Methods. Sixty rats were randomly divided into three groups: group 1, control group; group 2, rats received CP in a dose of 7 mg/kg/day for 5 consecutive days; group 3, CP was injected at 7 mg/kg per day for 5 consecutive days, and, on the 6th day of the experiment, rats were treated with SSC. Forty days after receiving the last dose of CP, rats were euthanized under anesthesia; testes were collected, and gene expression using real-time polymerase chain reaction for P53, Bax, caspase 9 and cytochrome c, testicular histological findings and oxidative status were determined. Results. Administration of cisplatin caused significant increases in malondialdehyde levels, Box and caspase 9 genes expression levels concomitant with significant decreases in anti-oxidant enzyme activities, p53 and cytochrome c gene expression levels, along with some histopathological lesions in testicular tissue. SCC attenuated the disturbance in oxidant/anti-oxidant status and testicular apoptosis; this is associated with improvements in the histopathological view of the testicular tissue. Conclusions. The current study highlights evidence that the SCC has anti-oxidative and antiapoptotic properties that could reverse CP-induced testicular toxicity, in addition to their role in spermatogenesis.

• 出版日期2015-11