Genetic transformation of wheat: progress during the 1990s into the Millennium

作者：Ingram HM; Livesey NL; Power JB; Davey MR^*

来源：Acta Physiologiae Plantarum, 2001, 23(2): 221-239.

DOI：10.1007/s11738-001-0012-2

• Agrobacterium tumefaciens
• biolistics
• gene expression
• microprojectile bombardment
• transformation
• wheat (Triticum aestivum L.)

Wheat transformation technology has progressed rapidly during the past decade. initially, procedures developed for protoplast isolation and culture, electroporation- and polyethylene glycol (PEG)-induced DNA transfer enabled foreign genes to be introduced into wheat cells. The development of biolistic (microprojectile) bombardment procedures led to a more efficient approach for direct gene transfer. More recently, Agrobacterium-mediated gene delivery procedures, initially developed for the transformation of rice, have also been used to generate transgenic wheat plants. This review summarises the considerable: progress in wheat transformation achieved during the last decade.
An increase in food production is essential in order to sustain the increasing world population. This could be achieved by the development of higher yielding varieties with improved nutritional quality and tolerance to biotic and abiotic stresses. Although conventional breeding will continue to play a major role in increasing crop yield, laboratory-based techniques, such as genetic transformation to introduce novel genes into crop plants, will be essential in complementing existing breeding technologies.
A decade ago, cereals were considered recalcitrant to transformation. Since then, a significant research effort has been focused on cereals because of their agronomic status, leading to improved genetic transformation procedures (Bommineni and Jauhnr 1997). initially, the genetic transformation of cereals relied on the introduction of DNA into protoplasts and the subsequent production of callus from which fertile plants were regenerated. More recently, major advances have been accomplished in the regeneration of fertile plants from a range of source tissues, providing an essential foundation for the generation of transgenic plants. This review summarises procedures, victors and target tissues used for transformation, highlights the limitations of current approaches and discusses future trends. The citation of references is limited, where possible, to the most relevant or recent reports.

• 出版日期2001