A tool for the selective sequestration of ATP and PPi to aid in-solution phosphopeptide detection assays

作者:Duodu Eugenia; Kraskouskaya Dziyana; Gomez Biagi Rodolfo F; Gunning Patrick T*
来源:Analyst, 2016, 141(3): 820-822.
DOI:10.1039/c5an01414j

摘要

The presence of small phospho-anions, such as PPi and ATP in protein samples often complicates the robust detection of phosphoproteins by metal-based chemosensors and receptors. We herein report the development of a bis(Zn2+-cyclen)-triethyl-benzene scaffold which can selectively sequester PPi and ATP without affecting the detection of a di-phosphorylated peptide by a ProxyPhos chemosensor.

  • 出版日期2016