Multipoint-Emitting Optical Fibers for Spatially Addressable In Vivo Optogenetics

作者：Pisanello Ferruccio^*; Sileo Leonardo; Oldenburg Ian A; Pisanello Marco; Martiradonna Luigi; Assad John A; Sabatini Bernardo L; De Vittorio Massimo

来源：Neuron, 2014, 82(6): 1245-1254.

DOI：10.1016/j.neuron.2014.04.041

Optical stimulation and silencing of neural activity is a powerful technique for elucidating the structure and function of neural circuitry. In most in vivo optogenetic experiments, light is delivered into the brain through a single optical fiber. However, this approach limits illumination to a fixed volume of the brain. Here a focused ion beam is used to pattern multiple light windows on a tapered optical fiber. We show that such fibers allow selective and dynamic illumination of different brain regions along the taper. Site selection is achieved by a simple coupling strategy at the fiber input, and the use of a single tapered waveguide minimizes the implant invasiveness. We demonstrate the effectiveness of this approach for multipoint optical stimulation in the mammalian brain in vivo by coupling the fiber to a microelectrode array and performing simultaneous extracellular recording and stimulation at multiple sites in the mouse striatum and cerebral cortex.

• 出版日期2014-6-18