Analogs of the CLV3 Peptide: Synthesis and Structure-Activity Relationships Focused on Proline Residues

作者：Kondo Tatsuhiko; Yokomine Kenjiro; Nakagawa Aya; Sakagami Youji^*

来源：Plant and Cell Physiology, 2011, 52(1): 30-36.

DOI：10.1093/pcp/pcq146

• Arabidopsis thaliana
• CLV3
• Peptide hormone
• Structure-activity relationship

CLAVATA3 (CLV3) is a plant peptide hormone in which the proline residues are post-translationally hydroxylated and glycosylated. CLV3 plays a key role in controlling the stem cell mass in the shoot meristem of Arabidopsis thaliana. In a previous report, we identified a dodecapeptide (MCLV3) from CLV3-overexpressing Arabidopsis calli; MCLV3 was the smallest functional peptide derived from the CLV3 precursor. Here, we designed a series of MCLV3 analogs in which proline residues were substituted with proline derivatives or N-substituted glycines (peptoids). Peptoid substitution at Pro9 decreased bioactivity without affecting specific binding to the CLV1-related protein in cauliflower membrane. These findings suggest that peptoid-substituted peptides would be lead compounds for developing potential agonists and antagonists of CLV3.

• 出版日期2011-1