DEVELOPMENT OF POLYMORPHIC MICROSATELLITE MARKERS FOR THE KILLARNEY FERN (VANDENBOSCHIA SPECIOSA, HYMENOPHYLLACEAE)

作者：del Carmen Garcia Lopez M; Ben Menni Schuler Samira; Lopez Flores Inmaculada; Nieto Lugilde Marta; Terron Camero Laura; Mazuecos Aguilera Ismael; Suarez Santiago Victor N^*

来源：Applications in Plant Sciences, 2015, 3(11): 1500067.

DOI：10.3732/apps.1500067

• endangered fern
• Hymenophyllaceae
• microsatellite markers
• population genetics
• Vandenboschia speciosa

Premise of the study: We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian-European species using DNA hypervariable markers.
Methods and Results: Ten primer sets were developed and tested on 47 individuals in a total of two Iberian populations of V. speciosa. The primers amplified di- and hexanucelotide repeats. The number of alleles ranged from two to eight, and the expected heterozygosity ranged from 0.107 to 0.807 among the populations analyzed.
Conclusions: The 10 microsatellite markers developed will be useful in characterizing the genetic diversity of V. speciosa and understanding its population structure (including the possible structure between sporophyte and gametophyte phases) and biogeographic history, and will provide important genetic data for the conservation of this species.

• 出版日期2015-11