Development and characterization of 12 microsatellite loci from the blueberry gall midge Dasineura oxycoccana (Diptera: Cecidomyiidae)

作者：Kim Hyojoong^*; Rodriguez Saona Cesar; Kwon Deok Ho; Park Sangwook; Kang Taek Jun; Kim Se Jin; Hong Ki Jeong; Lee Heung Sik

来源：Applied Entomology and Zoology, 2015, 50(3): 415-418.

DOI：10.1007/s13355-015-0335-x

• Exotic insect pest
• Biological invasion
• Microsatellite
• Population genetics
• SSR marker

The blueberry gall midge, Dasineura oxycoccana (Johnson), is a serious insect pest of blueberries and cranberries in North America. In this study, we developed 12 novel microsatellite loci for this gall midge. To obtain its sequence data, next-generation sequencing was performed using mixed individuals collected from Korea and the USA. The developed loci were polymorphic, with four to nine alleles in 20 individuals from a single population of New Jersey, USA. The analyses revealed that all 20 individuals had different multilocus genotypes with expected heterozygosity ranging from 0.708 to 0.920. In addition, we confirmed the discriminative resolution of all individuals collected. These markers will facilitate future studies on the genetic structure of blueberry gall midge populations.

• 出版日期2015-8
• 单位rutgers