The cellular antioxidant activity in red blood cells (CAA-RBC): A new approach to bioavailability and synergy of phytochemicals and botanical extracts

作者：B**** Manuela; Angelino Donato; Gennari Lorenzo; Ninfali Paolino^*

来源：Food Chemistry, 2011, 125(2): 685-691.

DOI：10.1016/j.foodchem.2010.09.065

• Flavonoids
• Botanical extracts
• Cellular antioxidant activity
• ORAC
• Erythrocytes
• Phytochemicals synergy
• Bioavailability

In the present work, human red blood cells (RBC) were used to determine cellular antioxidant activity (CAA-RBC) of pure phytochemicals and botanical extracts, with the aim to predict their bioavailability. Amongst the pure flavonoids, isorhamnetin, tamarixetin, myricetin, and kaempferol showed the highest activity in the CAA-RBC assay; whereas, with the "chemical" oxygen radical absorbance capacity (ORAC) assay, the compounds that showed the highest activity were isorhamnetin, resveratrol, apigenin and catechin. When the CAA-RBC assay was applied to herbal extracts, the Vitis vinifera showed the highest value, a position that this extract maintained also when the ORAC assay was used. Other extracts showed a different order of effectiveness with the two methods.
We also employed the CAA-RBC to assess synergistic or antagonistic effects of combinations of herbal extracts and we again compared the results with the ORAC assay. Punica granatum + Malus domestica synergized in the CAA-RBC assay, but not in the ORAC assay; Aspalathus linearis extract interacted positively with Vaccinium myrtillus, both in the ORAC assay and in the CAA-RBC assay. We concluded that the CAA-RBC assay, coupled with the ORAC assay, was useful for evaluating intracellular bioactivity and synergy amongst phytochemicals or extracts.

• 出版日期2011-3-15