Modification of Esperase (R) by covalent bonding to Eudragit (R) polymers L 100 and S 100 for wool fibre surface treatment

作者：Smith E; Zhang Q; Shen J^*; Schroeder M; Silva C

来源：Biocatalysis and Biotransformation, 2008, 26(5): 391-398.

DOI：10.1080/10242420802249521

• Chemical modification
• Eudragit (R)
• protease
• Esperase (R)

The protease Esperase(R) was modified by covalent bonding with two grades of a reversible soluble-insoluble co-polymer of methacrylic acid and methyl-methacrylate, namely Eudragit(R) L 100 and Eudragit(R) S 100. The optimum reaction conditions and washing protocol were investigated and it was found that Esperase(R) modified with Eudragit(R) L 100 showed greater activity than if modified with Eudragit(R) S 100. This should be expected as there is a greater quantity of active sites, namely carboxyl groups, per mass of Eudragit(R) L 100 in comparison with Eudragit(R) S 100 to interact with the enzyme. Gel filtration confirmed that Eudragit(R) L 100 covalently bonded to Esperase(R). Treatment of the modified Esperase(R) on wool showed that the enzyme modified with Eudragit(R) L 100 had greater activity towards the wool and appeared more effective in shrink resistant finishing.

• 出版日期2008