Hydroxysafflor yellow A suppresses oxidized low density lipoprotein induced proliferation of vascular smooth muscle cells

作者：Sheng Lin^*; Bi Shaojie; Cheng Chao; Zhang Jingbo

来源：Bangladesh Journal of Pharmacology, 2012, 7(2): 87-93.

DOI：10.3329/bjp.v7i2.10499

• ERK1/2
• Hydroxysafflor yellow A
• MAKP-1
• Oxidized low density lipoprotein
• Smooth muscle cells

To investigate the relationship between the suppression of Hydroxysafflor yellow A (HSYA) on the oxidized low density lipoprotein (ox-LDL) induced proliferation of vascular smooth muscle cells (VSMCs) and the mRNA and protein expression of extracellular signal-regulated protein kinase 1/2 (ERK1/2) and mitogen activated protein kinase phospholipase-1 (MAKP-1), VSMCs were treated with HSYA at 10 mu mol/L and/or ox-LDL at 35 mg/L for 48 h. MTT assay was done to measure cell survival rate, flow cytometry to detect cell cycle, reverse transcription PCR and Western blot to detect the expression of ERK1/2 and MAKP-1. When compared to cells treated with ox-LDL alone, the survival rate of cells treated with two reagents was reduced and the proportion of cells in G(0)/G(1) phase significantly increased, with increased MKP-1 expression. The study suggests HSYA can inhibit VSMC proliferation via increasing MKP-1 expression, reducing p-ERK1/2 activity and suppressing cell cycle.

• 出版日期2012
• 单位山东大学