Dietary supplementation with omega-3 fatty acid attenuates 5-fluorouracil induced mucositis in mice

作者：Generoso Simone de Vasconcelos; Rodrigues Nubia Morais; Trindade Luisa Martins; Paiva Nivea Carolina; Cardoso Valbert Nascimento; Carneiro Claudia Martins; de Matos Ferreira Adaliene Versiani; Caetano Faria Ana Maria; Maioli Tatiani Uceli

来源：Lipids in Health and Disease, 2015, 14(1): 54.

DOI：10.1186/s12944-015-0052-z

• Omega-3 fatty acid
• Mucositis
• Intestinal damage

Background: Studies showed the positive effects of omega-3 fatty acid (n-3 FA) for the treatment of inflammatory bowel disease as it alleviated the symptoms and promoted better mucosal integrity. The objective of this study was to determine whether a diet with the addition of n-3 FA helps control the inflammation observed in 5-fluorouracil (5-FU) induced mucositis.
Methods: BALB/c mice were randomly divided into four groups as follows: 1: control (CTL), fed a standard chow diet; 2: CTL + n-3 FA - n-3 FA, fed a diet with n-3; 3: mucositis (MUC), fed a standard chow diet and subjected to mucositis; and 4: MUC+ n-3 FA, fed a diet with n-3 FA and subjected to mucositis. On the 8th day, the animals of the MUC and MUC + n-3 FA groups received an intraperitoneal injection of 300 mg/kg 5-FU for mucositis induction. After 24 h or 72 h, all mice were euthanized and evaluated for intestinal permeability, bacterial translocation, intestinal histology and apoptosis.
Results: Mice that received the diet with n-3 FA and a 5-FU injection showed less weight loss compared to the animals of the MUC group (p < 0.005). Decreased intestinal permeability and bacterial translocation were also observed in animals fed n-3 FA, and these mice underwent mucositis compared to the MUC group (p < 0.005). These data were associated with mucosal integrity and a reduced number of apoptotic cells in the ileum mucosa compared to the mice that received the control diet and 5-FU injection.
Conclusion: Together, these results show that omega-3 fatty acid decreases the mucosal damage caused by 5-FU-induced mucositis.

• 出版日期2015-6-12