Detection of Rhopalosiphum insertum (apple-grass aphid) predation by the predatory mite Anystis baccarum using molecular gut analysis

作者：Cuthbertson AGS^*; Fleming CC; Murchie AK

来源：Agricultural and Forest Entomology, 2003, 5(3): 219-225.

DOI：10.1046/j.1461-9563.2003.00181.x

• Anystis baccarum
• aphid
• gut contents
• PCR
• predation
• Rhopalosiphum insertum

1 A simple, yet sensitive polymerase chain reaction based technique was developed for the detection of the apple-grass aphid Rhopalosiphum insertum in the gut of Anystis baccarum, a predatory mite.
2 A range of conserved polymerase chain reaction primers for insect mitochondrial and ribosomal DNA were tested in order to amplify R. insertum DNA. The mitochondrial DNA primers LrRNAR2+N1F1, amplified a region between the ND1 and large subunit RNA genes.
3 DNA sequencing of the R. insertum ND1-LRNA polymerase chain reaction product allowed aphid-specific polymerase chain reaction primers to be designed. These amplified a 283-bp product from individual aphids. No polymerase chain reaction product was amplified from individual A. baccarum.
4 Using the aphid-specific primers against A. baccarum fed on R. insertum, the diagnostic 283-bp product was amplified.
5 Two restriction enzymes (RsaI and AluI) produced patterns that allowed unambiguous identification of R. insertum DNA from that of Macrosiphum euphorbiae and Myzus persicae.

• 出版日期2003-8