Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations

作者:Kalyanaraman Balaraman*; Darley Usmar Victor; Davies Kelvin J A; Dennery Phyllis A; Forman Henry Jay; Grisham Matthew B; Mann Giovanni E; Moore Kevin; Roberts L Jackson II; Ischiropoulos Harry
来源:Free Radical Biology and Medicine, 2012, 52(1): 1-6.
DOI:10.1016/j.freeradbiomed.2011.09.030

摘要

The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results.

  • 出版日期2012-1-1