Anti-Inflammatory Bioactivities of Honokiol through Inhibition of Protein Kinase C, Mitogen-Activated Protein Kinase, and the NF-kappa B Pathway To Reduce LPS-Induced TNF alpha and NO Expression

作者：Chao Louis Kuoping; Liao Pei Chun; Ho Chen Lung; Wang Eugene I Chen; Chuang Chao Chin; Chiu Huan Wen; Hung Lang Bang^*; Hua Kuo Feng

来源：Journal of Agricultural and Food Chemistry, 2010, 58(6): 3472-3478.

DOI：10.1021/jf904207m

• Honokiol
• LPS
• cytokines
• signaling

Much recent research has demonstrated that honokiol, a phenolic compound originally isolated from Magnolia officinalis, has potent anticancer activities; however, the detailed molecular mechanism of its anti-inflammatory activity has not yet been fully addressed. In this study we demonstrated that honokiol inhibited lipopolysaccharide (LPS)-induced tumor necrosis factor-alpha. secretion in macrophages, without affecting the activity of the tumor necrosis factor-a converting enzyme. At the same time, honokiol not only inhibited nitric oxide expression in LPS-stimulated murine macrophages but also inhibited the LPS-induced phosphorylation of ERK1/2, JNK1/2, and p38. By means of confocal microscope analysis we demonstrated that phosphorylation and membrane translocation of protein kinase C-alpha, as well as NF-kappa B activation, were inhibited by honokiol in LPS-stimulated macrophages. Furthermore, it was found that honokiol neither antagonizes the binding of LPS to cells nor alters the cell surface expression of toll-like receptor 4 and CD14. Our current results have exhaustively described the anti-inflammatory properties of honokiol, which could lead to the possibility of its future pharmaceutical application in the realm of immunomodulation.

• 出版日期2010-3-24
• 单位中国医科大学