Development of a Novel Jet Detection Cell for Electrochemiluminescence Analysis

作者：Xi, Qiang; Chen, Yu; Liu, Zhongming; Wang, Jie^*

来源：Analytical Letters, 2015, 48(13): 2022-2030.

DOI：10.1080/00032719.2015.1010119

• Tris(2,2 '-bipyridyl)ruthenium(II)
• Electrochemiluminescence
• N-Butyldiethanolamine
• Jet detection cell

A new type of jet detection cell for electrochemiluminescence (ECL) analysis was designed in which samples are jetted on an electrode through a small, fixed steel needle on the front of the working electrode. Under the optimal conditions, the logarithm of electrochemiluminescence intensity was directly proportional to the concentration of tris(2,2-bipyridyl)ruthenium(II) from 10(-10) to 10(-5) molar with a limit of detection equal to 5x10(-11) molar. The relative standard deviation for 10(-8) molar
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was 1.2 percent for eleven replicate measurements. The analytical figures of merit were improved compared to conventional static detection and the detection limit of
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was reduced by approximately one order of magnitude. This method offers low sample consumption, rapid analysis, and easy cleaning, making it suitable for immunoassays and DNA assays.

• 出版日期2015-9-2
• 单位广州军区广州总医院; 华南理工大学