Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors

作者：Lee Liang Ming; Lin Chia Ho; Chung Hsien Hui; Cheng Juei Tang; Chen I Hung; Tong Yat Ching^*

来源：Lower Urinary Tract Symptoms, 2013, 5(1): 39-43.

DOI：10.1111/j.1757-5672.2012.00158.x

• agmatine
• adenosine-5'-triphosphate-sensitive K plus channel
• imidazoline receptor
• prostate

Objectives: The effect of agmatine on prostate contractility as well as the roles of imidazoline receptors and potassium channels in this action were studied using isolated Wistar rat prostate tissue. Methods: Rat prostate strips were pre-contracted with 1 mu mol/L phenylephrine or 50 mmol/L KCl. The relaxation response to agmatine (1100 mu mol/L) was measured. The effects of imidazoline receptor blockers: efaroxan, BU224, KU14R; ATP-sensitive K+ channels (KATP) channel inhibitor: glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 on the agmatine-induced relaxation were studied. Results: Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner. This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R respectively). Moreover, the agmatine-induced relaxation was attenuated by glibenclamide and H-89, but enhanced by IBMX. Conclusion: The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.

• 出版日期2013-1