Aliidiomarina iranensis sp nov., a haloalkaliphilic bacterium from a coastal-marine wetland

作者：Amoozegar Mohammad Ali^*; Shahinpei Azadeh; Fazeli Seyed Abolhassan Shahzadeh; Schumann Peter; Sproer Cathrin; Ventosa Antonio

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(5): 2099-2105.

DOI：10.1099/ijsem.0.000996

A novel Gram-stain-negative, straight rod-shaped, non-pigmented, slightly halophilic and alkaliphilic bacterium, designated strain GBPy7(T), was isolated from a sample of the coastal-marine wetland Gomishan in Iran. Cells of strain GBPy7(T) were motile. Growth occurred on media with 1-15 % (w/v) NaCl (optimum 3 %), at pH 7-10 (optimum pH 8.5) and at 4-45 degrees C (optimum 37 degrees C). Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that strain GBPy7(T) belonged to the family Idiomarinaceae. Its closest relatives were Aliidiomarina shirensis AIS(T) (98.1 % 16S rRNA gene sequence similarity) and other Aliidiomarina species (95.9-94.2 %), together with Idiomarina seosinensis CL-SP19(T) (94.3 %) and Idiomarina fontislapidosi F23(T) (94.3 %). The major cellular fatty acids of the isolate were iso-C-15 : 0, iso-C-17 : 0, iso-C-17 : 1 omega 9c and C-18 : 1 omega 7c and its polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown aminophospholipid. Cells of strain GBPy7(T) contained ubiquinone Q-8. The G+C content of the genomic DNA of this strain was 51.6 mol%. The level of DNA-DNA relatedness between strain GBPy7(T) and A. shirensis IBRC-M 10414(T) was 21 %. The physiological, biochemical, genotypic and phylogenetic differences between strain GBPy7(T) and other previously described taxa indicate that the strain represents a novel species of the genus Aliidiomarina within the family Idiomarinaceae, for which the name Aliidiomarina iranensis sp. nov. is proposed. The type strain is GBPy7(T) (=IBRC-M 10763(T) =CECT 8339(T)).

• 出版日期2016-5