A modified electro-transformation method for Bacillus subtilis and its application in the production of antimicrobial lipopeptides

作者：Cao, Guoqiang; Zhang, Xiaohui; Zhong, Lei; Lu, Zhaoxin^*

来源：Biotechnology Letters, 2011, 33(5): 1047-1051.

DOI：10.1007/s10529-011-0531-x

• Antimicrobial lipopeptides
• Bacillus subtilis
• Electroporation
• Trehalose

A modified electroporation method using trehalose is presented for the transformation of Bacillus subtilis. The new method improved the transformation efficiency of B. subtilis nearly 2,000-fold compared with the usual method, giving 4 x 10(5) transformants/mu g DNA. Using this method, B. subtilis was engineered to improve production of antimicrobial lipopeptides and produced 1.8-fold more surfactin and 2.9-fold more fengycin.

• 出版日期2011-5
• 单位南京农业大学