A Collagen alpha 2(I) Mutation Impairs Healing after Experimental Myocardial Infarction

作者：Hofmann Ulrich^*; Bonz Andreas; Frantz Stefan; Hu Kai; WalIer Christiane; Roemer Katrin; Wolf Juergen; Gattenloehner Stefan; Bauersachs Johann; Ertl Georg

来源：American Journal Of Pathology, 2012, 180(1): 113-122.

DOI：10.1016/j.ajpath.2011.09.033

Collagen breakdown and de novo synthesis are important processes during early wound healing after myocardial infarction (MI). We tested the hypothesis that collagen I, the main constituent of the extracellular matrix, affects wound healing after MI. The osteogenesis imperfecta mouse (OIM), lacking procollagen-a2(I) expression, represents a model of the type III form of the disease in humans. Homozygous (OIM/OIM), heterozygous (OIM/WT), and wild-type (WT/WT) mice were subjected to a permanent myocardial infarction protocol or sham surgery. Baseline functional and geometrical parameters determined by echocardiography did not differ between genotypes. After MI but not after sham surgery, OIM/OIM animals exhibited significantly increased mortality, due to early ventricular rupture between day 3 and 7. Echocardiography at day 1 demonstrated increased left ventricular dilation in OIM/OIM animals. Less collagen I mRNA within the infarct area was found in OIM/OIM animals. At 2 days after MI, MMP-9 expression in the infarct border zone was higher in OIM/OIM than in WT/WT animals. Increased granulocyte infiltration into the infarct border zone occurred in OIM/OIM animals. Neither granulocyte depletion nor MMP inhibition reduced mortality in OIM/OIM animals. In this murine model, deficiency of collagen I leads to a myocardial wound-healing defect. Both structural alterations within pre-existing collagen matrix and impaired collagen de novo expression contribute to a high rate of early myocardial rupture after MI. (Am J Pathol 2012, 180:113-122; DOI: 10.1016/j.ajpath.2011.09.033)

• 出版日期2012-1