A non-enzymatic nanomagnetic electro-immunosensor for determination of Aflatoxin B-1 as a model antigen

作者：Masoomi Leila; Sadeghi Omid; Banitaba Mohammad Hossein; Shahrjerdi Akram; Davarani Saied Saeed Hosseiny^*

来源：Sensors and Actuators B: Chemical , 2013, 177: 1122-1127.

DOI：10.1016/j.snb.2012.11.067

• Magnetic nanocomposites
• Sandwich biosensor
• Electrochemical impedance spectroscopy
• Aflatoxin B-1
• Nonenzymatic electrochemical immunosensors

A nonenzymatic sandwich-type electrochemical immunosensor was developed by means of a modified glassy carbon electrode: GCE/chitosan/Au nanoparticle (AuNP)/anti-Aflatoxin B-1 (a AFB(1)), and a Fe3O4 magnetic core with a gold shell functionalized with 3-((2-mercaptoethylimino)methyl)benzene-1,2-diol and labeled with an AFB(1). The immunosensor was used for the detection of Aflatoxin B-1 as a model antigen. The current originated from the conversion of catechol to o-quinone and vice versa within a quasi-reversible two electrons-two protons reaction, considered as the response. The electrochemical studies demonstrated that the proposed immunoelectrode can be used to detect AFB(1) in the range of 0.6-110 ng mL(-1) with a detection limit of 0.2 ng mL(-1). This immunosensor can be simply regenerated by using an external magnetic field which ensures a reproducible response.

• 出版日期2013-2