Mannotriose regulates learning and memory signal transduction in the hippocampus(star star star star)

作者：Zhang, Lina; Dai, Weiwei; Zhang, Xueli; Gong, Zhangbin; Jin, Guoqin^*

来源：Neural Regeneration Research, 2013, 8(32): 3020-3026.

DOI：10.3969/j.issn.1673-5374.2013.32.005

• neural regeneration
• traditional Chinese medicine
• Rehmannia
• mannotriose
• corticosterone
• hippocampus
• neurons
• learning and memory
• grants-supported paper
• neuroregeneration

Rehmannia is a commonly used Chinese herb, which improves learning and memory. However, the crucial components of the signal transduction pathway associated with this effect remain elusive. Primary hippocampal neurons were cultured in vitro, insulted with high-concentration (1 x 10(-4) mol/L) corticosterone, and treated with 1 x 10(-4) mol/L mannotriose. Thiazolyl blue tetrazolium bromide assay and western blot analysis showed that hippocampal neuron survival rates and protein levels of glucocorticold receptor, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor were all dramatically decreased after high-concentration corticosterone-induced injury. This effect was reversed by mannotriose, to a similar level as RU38486 and donepezil. Our findings indicate that mannotriose could protect hippocampal neurons from high-concentration corticosterone-induced injury. The mechanism by which this occurred was associated with levels of glucocorticoid receptor protein, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor.

• 出版日期2013-11
• 单位上海中医药大学