Determination of trifolirhizin in rat plasma by UPLC: Application to a pharmacokinetic study

作者：Ni Kong hai; Wen Zheng de; Huang Xin ce; Wang Chen xi; Ye Tian tian; Hu Guo xin; Zhou Meng tao^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2015, 990: 181-184.

DOI：10.1016/j.jchromb.2015.03.031

• Trifolirhizin
• Rat plasma
• Pharmacokinetic
• UPLC

In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography (UPLC) has been developed for the determination of trifolirhizin in rat plasma using pirfenidone as internal standard (IS). After sample preparation by a simple liquid-liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1 mm x 50 mm, 1.7 mu m particle size) and ultraviolet detection set at a wavelength of 366 nm. The method was linear over the concentration range 25-1000 ng/mL with a lower limit of quantification (LLOQ) of 25 ng/mL. Inter- and intra-day precision (RSD%) were all within 10.2% and the accuracy (RE%) was equal or lower than 9.3%. The recovery was in the range of 78.5-86.4% for trifolirhizin and 87.4% for IS. Stability studies showed that trifolirhizin was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study involving oral administration of trifolirhizin to rats.

• 出版日期2015-5-15
• 单位温州医科大学