alpha-Enolase binds to RNA

作者：Hernandez Perez Liliana; Depardon Francisco; Fernandez Ramirez Fernando; Sanchez Trujillo Alejandra; Maria Bermudez Cruz Rosa; Dangott Lawrence; Montanez Cecilia^*

来源：Biochimie, 2011, 93(9): 1520-1528.

DOI：10.1016/j.biochi.2011.05.007

• Alpha enolase
• CUG repeats
• RNA binding protein
• Mass spectrometry

To detect proteins binding to CUG triplet repeats, we performed magnetic bead affinity assays and North-Western analysis using a (CUG)(10) ssRNA probe and either nuclear or total extracts from rat L6 myoblasts. We report the isolation and identification by mass spectrometry and immunodetection of alpha-enolase, as a novel (CUG)n triplet repeat binding protein. To confirm our findings, rat recombinant alpha-enolase was cloned, expressed and purified; the RNA binding activity was verified by electrophoretic mobility shift assays using radiolabeled RNA probes. Enolase may play other roles in addition to its well described function in glycolysis.

• 出版日期2011-9