NMR investigations of interactions between anesthetics and lipid bilayers

作者：Castro Vasco; Stevensson Baltzar; Dvinskikh Sergey V; Hogberg Carl Johan; Lyubartsev Alexander P; Zimmermann Herbert; Sandstrom Dick; Maliniak Arnold^*

来源：Biochimica et Biophysica Acta-Biomembranes, 2008, 1778(11): 2604-2611.

DOI：10.1016/j.bbamem.2008.07.023

• Solid-state NMR
• Carbon-13
• Phosphorus-31
• Deuterium
• Dipolar couplings
• Separated local field
• Quadrupolar coupling
• Order parameter
• Lyotropic liquid crystal
• Biomembrane
• Anesthetic

Interactions between anesthetics (lidocaine and short chain alcohols) and lipid membranes formed by dimyristoylphosphatidylcholine (DMPC) were studied using NMR spectroscopy. The orientational order of lidocaine was investigated using deuterium NMR on a selectively labelled compound whereas segmental ordering in the lipids was probed by two-dimensional (1)H-(13)C separated local field experiments under magic-angle spinning conditions. In addition, trajectories generated in molecular dynamics (MD) computer simulations were used for interpretation of the experimental results. Separate simulations were carried out with charged and uncharged lidocame molecules. Reasonable agreement between experimental dipolar interactions and the calculated counterparts was observed. Our results clearly show that charged lidocaine affects significantly the lipid headgroup. In particular the ordering of the lipids is increased accompanied by drastic changes in the orientation of the P-N vector in the choline group.

• 出版日期2008-11
• 单位河北医科大学