Anti-inflammatory effect of sargachromanol G isolated from Sargassum siliquastrum in RAW 264.7 cells

作者：Yoon Weon Jong; Heo Soo Jin; Han Sang Chul; Lee Hye Ja; Kang Gyeoung Jin; Kang Hee Kyoung; Hyun Jin Won; Koh Young Sang; Yoo Eun Sook^*

来源：Archives of Pharmacal Research, 2012, 35(8): 1421-1430.

DOI：10.1007/s12272-012-0812-5

• Sargachromanol G
• Sargassum siliquastrum
• Inflammatory markers
• Pro-inflammatory cytokines
• NF-kappa B
• MAPK

A study on the anti-inflammatory activity of brown alga Sargassum siliquastrum led to the isolation of sargachromanol G (SG). In this study, the anti-inflammatory effect and the action mechanism of SG have been investigated in murine macrophage cell line RAW 264.7. SG dosedependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E-2 (PGE(2)), and cyclooxygenase-2 (COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS-induced nuclear factor-kappa B (NF-kappa B) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. SG inhibited the phosphorylation I kappa B-alpha and NF-kappa B (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-kappa B activation and MAPK phosphorylation.

• 出版日期2012-8
• 单位河北医科大学