A novel voltammetric sensor for nevirapine, based on modified graphite electrode by MWCNs/poly(methylene blue)/gold nanoparticle

作者：Gholivand Mohammad Bagher^*; Ahmadi Elahe; Haseli Mozhdeh

来源：Analytical Biochemistry, 2017, 527: 4-12.

DOI：10.1016/j.ab.2017.03.018

• Nevirapine
• Stripping voltammetry
• Methylene blue
• Electro-polymerization
• Gold nanoparticles

In the present study, a graphite electrode (GE) modified by conductive film (containing functionalized multi-walled carbon nanotubes (f-MWCNTs), poly methylene blue p(MB) and gold nanoparticles (AuNPs)) was introduced for determination of nevirapine (NVP) as an anti-HIV drug by applying the differential pulse anodic stripping voltammetry (DPASV) technique. Modification of the electrode was investigated by scanning electron microscopy (SEM) and impedance electrochemical spectroscopy (EIS). All electrochemical effective parameters on detection of NVP were optimized and the oxidation peak current of drug was used for its monitoring. The obtained results confirmed that the oxidation peak currents increased linearly by increasing in NVP concentrations in the range of 0.1-50 mu M and a detection limit of 53 nM was achieved. The proposed sensor (AuNPs/p(MB)/f-MWCNTs/GE) was successfully applied for the determination of NVP in blood serum and pharmaceutical samples. It revealed the excellent stability, repeatability and reproducibility as well.

• 出版日期2017-6-15