Use of quantitative PCR technique for determining gene copy number in the genome of Beauveria bassiana transformant

作者：Dong Wei Xia; Feng Ming Guang; Ying Sheng Hua^*

来源：Journal of Asia-Pacific Entomology, 2017, 20(1): 57-59.

DOI：10.1016/1.aspen.2016.12.001

• Entomopathogenic fungus
• Genomic DNA
• Rapid detection
• Reference strain

This study describes the use of quantitative PCR (qPCR) to determine gene copy number in Beauveria bassiana genome. This procedure has been improved by generating a reference strain to calibrate PCR amplification efficiency and using a gamma-tubulin gene as internal control. The reference strain was constructed by introducing one copy of phosphinothricin resistance gene and two copies of green fluorescent protein gene into the wild-type strain. Finally, this improved procedure was validated via determining the gene copy number in the previously characterized transformants, and displays a great potential in rapid detection of gene copy number in the genome of transformant.

• 出版日期2017-3
• 单位浙江大学