Neutral Red versus MTT assay of cell viability in the presence of copper compounds

作者：Perez Mariela Gomez; Fourcade Lyvia; Mateescu Mircea Alexandru; Paquin Joanne^*

来源：Analytical Biochemistry, 2017, 535: 43-46.

DOI：10.1016/j.ab.2017.07.027

• Amino acid copper complexes
• Urea copper complex
• Copper salts
• MTT assay
• Neutral Red assay
• Neuron viability

Copper is essential for numerous physiological functions, and copper compounds may display therapeutic as well as cytotoxic effects. The MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay is a standard test largely used in cytotoxicity studies. This report shows that low micromolar levels of copper compounds such as Cu(II)Urea(2), Cu(II)Ser(2) and CuCl2 can interfere with the MTT assay making improper the detection of formazan product of KIT reduction. Comparatively, the Neutral Red assay appears to be sensitive and showing no interference with these compounds. The lactate dehydrogenase alternative assay cannot be used because of inhibitory effect of these copper compounds on the enzyme activity.

• 出版日期2017-10-15