Augmented cellular uptake and antiproliferation against pancreatic cancer cells induced by targeted curcumin and SPION encapsulated PLGA nanoformulation

作者：Sivakumar Balasubramanian; Aswathy Ravindran Girija; Nagaoka Yutaka; Iwai Seiki; Hasumura Takashi; Venugopal Kizhikkilot; Kato Kazunori; Yoshida Yasuhiko; Maekawa Toru; Sakthikumar Dasappan Nair^*

来源：Materials Express, 2014, 4(3): 183-195.

DOI：10.1166/mex.2014.1160

• Nanotechnology
• SPIONs
• Curcumin
• Pancreatic Cancer
• PLGA Nanoparticles
• Targeted Drug Delivery

Superparamagnetic iron oxide nanoparticles (SPIONs) employed theragnostic work in cancer therapy exploited its inherent magnetic properties that can be utilized for drug targeting and for drug delivery applications. To attain theragnostic properties, SPION and curcumin encapsulated HER2 targeted PLGA (HER2-CUR-SPION-PLGA) nano-formulation was developed by solvent evaporation technique (CUR-SPION-PLGA NPs). The physico-chemical properties of nanoconjugate were characterized using various instruments such as TEM, SEM, AFM, XPS, and VSM etc. The uptake of NPs was studied in pancreatic cancer cells (PANC-1 and MIA PaCa-2) and normal fibroblast cells (L929) by confocal microscopy and flowcytometry analysis. The anticancer potential of the NPs in the absence and presence of an external magnet (magnetic targeting property) was studied. The effect of curcumin on mitochondrial membrane potential integrity leading to apoptosis was also determined. The conjugation of HER2 to nanoconjugate for active magnetic targeting for the co-delivery of curcumin and MNPs enhanced synergistic therapeutic index. HER2-CUR-SPION-PLGA NPs displayed promising potential anticancer activity by destroying pancreatic cancer cells.

• 出版日期2014-6