Mechanism of action of the stimulatory effect of apigenin-6-C-(2 ''-O-alpha-L-rhamnopyranosyl)-beta-L-fucopyranoside on C-14-glucose uptake

作者：Cazarolli Luisa Helena; Folador Poliane; Moresco Henrique Hunger; Costa Brighente Ines Maria; Pizzolatti Moacir Geraldo; Mena Barreto Silva Fatima Regina

来源：Chemico-Biological Interactions, 2009, 179(2-3): 407-412.

DOI：10.1016/j.cbi.2008.11.012

• Flavonoid
• Glucose uptake
• Insulin
• Diabetes

There has been a growing interest in hypoglycemic agents from natural products, particularly those derived from plants. Flavonoids are naturally occurring phenolic compounds with a broad range of biological activities and the beneficial effects of flavonoids have been studied in relation to diabetes mellitus, either through their capacity to avoid glucose absorption or to improve glucose tolerance. The purpose of this study was to investigate the mechanism of action of the stimulatory effect of apigenin-6-C-(2"-O-alpha-L-rhamnopyranosyl)-beta-L-fucopyranoside (1), isolated from Averrhoa carambola L. (Oxalidaceae) leaves, on C-14-glucose uptake. This compound (1) was found to have an acute effect on blood glucose lowering in diabetic rats and stimulated glucose-induced insulin secretion after oral treatment in hyperglycemic rats. A significant stimulatory effect of compound 1 on 14C-glucose uptake was observed at 50 and 100 mu M. The effect of compound 1 on glucose uptake was completely nullified by wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), RO318220, an inhibitor of protein kinase C (PKC), PD98059, a specific inhibitor of mitogen-activated protein kinase (MEK), cycloheximide, an inhibitor of protein synthesis, and colchicine, a microtubule-depolymerizing agent. Compound 1 (100 mu M) and insulin (10 nM) did not show any synergistic effect on glucose uptake. These results suggest that the flavonoid may have a dual target of action, as an insulin-secretagogue and also as an insulin-mimetic agent.

• 出版日期2009-5-15