APC/C(Fzr/Cdh1) promotes cell cycle progression during the Drosophila endocycle

作者：Narbonne Reveau Karine; Senger Stefania; Pal Margit; Herr Anabel; Richardson Helena E; Asano Maki; Deak Peter; Lilly Mary A^*

来源：Development, 2008, 135(8): 1451-1461.

DOI：10.1242/dev.016295

• APC/C
• cdh1
• cyclin E
• Drosophila
• endoreplication
• endocycle
• Fzr/Cdh1
• geminin

The endocycle is a commonly observed variant cell cycle in which cells undergo repeated rounds of DNA replication with no intervening mitosis. How the cell cycle machinery is modified to transform a mitotic cycle into endocycle has long been a matter of interest. In both plants and animals, the transition from the mitotic cycle to the endocycle requires Fzr/Cdh1, a positive regulator of the Anaphase-Promoting Complex/Cyclosome (APC/C). However, because many of its targets are transcriptionally downregulated upon entry into the endocycle, it remains unclear whether the APC/C functions beyond the mitotic/endocycle boundary. Here, we report that APC/C(Fzr/Cdh1) activity is required to promote the G/S oscillation of the Drosophila endocycle. We demonstrate that compromising APC/C activity, after cells have entered the endocycle, inhibits DNA replication and results in the accumulation of multiple APC/C targets, including the mitotic cyclins and Geminin. Notably, our data suggest that the activity of APC/CFzr/Cdh1 during the endocycle is not continuous but is cyclic, as demonstrated by the APC/C-dependent oscillation of the pre-replication complex component Orc1. Taken together, our data suggest a model in which the cyclic activity of APC/C(Fzr/Cdh1) during the Drosophila endocycle is driven by the periodic inhibition of Fzr/Cdh1 by Cyclin E/Cdk2. We propose that, as is observed in mitotic cycles, during endocycles, APC/C(Fzr/Cdh1) functions to reduce the levels of the mitotic cyclins and Geminin in order to facilitate the relicensing of DNA replication origins and cell cycle progression.

• 出版日期2008-4-15