A novel gene that is up-regulated during recovery from cold shock in Drosophila melanogaster

作者：Goto SG^*

来源：Gene, 2001, 270(1-2): 259-264.

DOI：10.1016/S0378-1119(01)00465-6

• Frost
• proline-rich region
• signal peptide

Gene expression during recovery at 25 degreesC (rearing temperature) after cold shock (0 degreesC) was studied in Drosophila melanogaster using a subtraction technique. A novel gene (Frost, abbreviated as Fst) was considerably up-regulated during recovery after cold shock. In addition, a prolongation of cold shock was more effective for induction. In contrast to cold shock, Fst gene did not respond to heat shock. This gene is apparently the same as the unidentified gene, CG9434. Fst has high internal repeats not only in nucleotide but also in amino acid sequences. In addition, FST protein has a proline-rich region. The deduced amino acid sequence revealed a modular structure; i.e., a signal peptide in the N-terminal region followed by a long hydrophilic region. Therefore, this protein is likely to be directed into ER and secreted into extracellular space.

• 出版日期2001-5-30