Anti-neuroinflammatory Effects of Ethanol Extract of Inula helenium L (Compositae)

作者：Lee Sung Gyu; Kang Hyun^*

来源：Tropical Journal of Pharmaceutical Research, 2016, 15(3): 521-526.

DOI：10.4314/tjpr.v15i3.13

• Inula helenium
• DPPH
• Neurodegenerative diseases
• Anti-inflammatory
• Anti-oxidant
• Microglial cell

Purpose: To evaluate the in vitro antioxidant and anti-neuroinflammatory activities of Inula helenium extract (IHE) against lipopolysaccharide (LPS)-induced production of nitric oxide (NO) by primary microglial cells.
Methods: Cell viability was estimated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Antioxidant activity was evaluated using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. LPS-stimulated BV-2 microglia were used to study the expression and production of inflammatory mediators, including NO, inducible NO synthase (iNOS) and Interukin-6 (IL-6).
Results: Pretreatment with IHE prior to LPS treatment significantly inhibited excessive production of NO (p < 0.001 at 20, 40, 80 and 100 mu g/mL) in a dose-dependent manner, and was associated with downregulation of expression of inducible nitric oxide synthase (iNOS). IHE also suppressed the LPS-induced increase in IL-6 level (p < 0.01 at 40 and 80 mu g/mL) in BV-2 cells. The antioxidant activity exhibited by IHE might play a critical role in ameliorating neuroinflammatory processes in LPS-stimulated BV-2 microglial cells.
Conclusion: IHE may be beneficial in preventing and treating neurodegenerative and oxidative stressrelated diseases.

• 出版日期2016-3