Application of liguid isoelectric focusing combined acetylation of stable-isotope labeling technique in comparative proteomics

作者：Li Na^*; Yang Peng Yuan

来源：Chinese Journal of Analytical Chemistry, 2008, 36(4): 449-453.

• liquid isoelectric focusing
• SDSPAGE
• acetylation labeling
• proteomics

A new method of quantitative proteomics which combines free solution-isoelectric focusing, SDSPAGE and acetylation of stable-isotope labeling techniques was developed. This technology has good dynamic range between 1:5 similar to 5:1 (less than 20%). Compared to the traditional comparative proteomics, this method not only improves the throughput of comparative proteomics but also help to find out the differentially expressed proteins in the low abundance and alkaline proteins. This technology have been applied to investigate the comparative proteomics of human liver cancer. 16 differentially expressed proteins were identified; some of these proteins have been reported to be related with liver cancer.

• 出版日期2008-4
• 单位复旦大学