Deuterium isotope effects in oxidation of dopamine by tyramine oxidase

作者：Pajak Malgorzata; Byszewska Wioleta; Kanska Marianna^*

来源：Nukleonika, 2013, 58(2): 255-259.

• deuterium
• dopamine (DA)
• isotope effects
• oxidation
• tyramine oxidase

We report the studies on the mechanism of oxidation of dopamine (DA) to 3%26apos;,4%26apos;-dihydroxyphenylacetaldehyde (DOPAL) by enzyme tyramine oxidase (EC 1.4.3.6) using kinetic isotope effects (ME) and solvent isotope effects (SIB) methods. For kinetic studies, the selectively deuterium labelled isotopologues of dopamine, i.e., [(1R)-H-2]-, and [(1S)-H-2]-DA were used. The numerical values of KIE%26apos;s for the (1R)- and (1S)-isotopologues of dopamine in the enzymatic oxidation of DA to DOPAL were determined using the non competitive spectrophotometric method. Also, the SIB%26apos;s for this reaction carried out in heavy water were obtained spectrophotometrically. Some mechanistic details of enzymatic oxidation of DA to DOPAL were discussed.

• 出版日期2013