DEGRADATION CHARACTERISTICS AND PATHWAY OF FENPROPATHRIN BY Rhodopseudomonas sp STRAIN PSB07-6

作者:Zhang, Songbai; Zhang, Deyong; Liu, Yong*; Luo, Xiangwen; Cheng, Feixue; Luo, Yuanhua; Cheng, Ju'e; Ma, Xiaoming
来源:Fresenius Environmental Bulletin, 2009, 18(11): 2060-2065.

摘要

To isolate, select, identify and assess the potential for the biodegradation of fenpropathrin by photosynthetic bacterium, fenpropathrin-degrading photosynthetic bacteria were isolated by enrichment culture. Five isolates, which showed fenpropathrin biodegradation activity, were screened. Of these, strain PSB07-6 exhibited the highest fenpropathrin biodegradation potential, and was selected for further study. this strain was identified as Rhodopseudomonas sp. on the basis of morphology, physiological and biochemical characteristics, and 16S rRNA sequence analysis. The characteristics of this strain for degrading fenpropathrin were performed under different culture conditions. Optimum activity occurred at 35 degrees C and pH 7. Strain PSB07-6 degraded 67.43% fenpropathrin (200 mg L-1) within 15 days. This strain could also degrade effectively cypermethrin, cyfluthrin, biphenthrin and esfenvalerate, except deltamethrin. Gas chromatography with mass selective detector (GC-MS) identified m-phenoxyphenyl-acetonitrile as the stable metabolic product, and from this a fenpropathrin biodegradation pathway is proposed.