Acetylsalicylic acid is compounding to antiplatelet effect of C-reactive protein

作者：Boncler Magdalena; Luzak Boguslawa; Rozalski Marcin; Golanski Jacek; Rychlik Blazej; Watala Cezary^*

来源：Thrombosis Research, 2007, 119(2): 209-216.

DOI：10.1016/j.thromres.2006.01.004

• acetylsalicylic acid
• C-reactive protein
• inflammation
• platelet activation

The contribution of inflammatory process to the modulation of platelet response to acetylsalicylic acid (ASA) remains obscure. In our study, we examined the in vitro effect of C-reactive protein (CRP) on the ASA-mediated inhibition of collagen-stimulated platelet reactivity. Influence of CRP on platelet responsiveness to ASA was analysed using classical turbidimetric aggregation and flow cytometry. When acting alone, both C-reactive protein and ASA inhibited collagen-dependent platelet aggregation and reduced the expressions of two platelet surface membrane activation markers: P-selectin and activated GPIIbIIIa complex. Compared to the effects observed for ASA alone, the simultaneous action of both agents lead to further reductions in platelet aggregation (by 56.7 +/- 1.0% vs. 14.9 +/- 0.6%, p < 0.0001) and lowered the expressions of platelet surface membrane P-setectin (by 72.1 +/- 5.3% vs. 65.0 +/- 6.0%, p < 0.01) and activated GPIIbIIIa (by 67.0 +/- 5.6% vs. 47.7 +/- 8.3%, p < 0.01). In general, our findings showed for the first time the augmenting effect of native C-reactive protein in the antiplatelet action of acetylsalicylic acid. Thus, we conclude that the effectiveness of aspirin therapy may strongly depend upon the presence of native CRIP in circulation.

• 出版日期2007