A "GC-rich" method for mammalian gene expression: A dominant role of non-coding DNA GC content in regulation of mammalian gene expression

作者：Jia Qian^*; Wu HongTao; Zhou XingJun; Gao Jian; Zhao Wei; JouDi, Aziz; Wei JingShuang; Hou LiHua; Wu ShuYin; Zhang Ying; Dong XiangFeng; Huang YanMin; Jin WeiYuan; Zhu HongJie; Zhao XinHui; Huang ChunHua; Xing LiPing; Li LiWen; Ma Jun; Liu XiYan; Tao Ran; Ye ShuaiDong; Song YiGao; Song LingLing; Chen GuanPing; Du ChunLing; Zhang XueTing; Li Bo; Wang YanTao; Yang Wei; Rishton, Gilbert; Teng YuYang; Leng GouQing; Li LuanFeng; Liu WenXian; Cheng LiJun; Liang QiuBo; Li ZhengWu

来源：Science China Life Sciences, 2010, 53(1): 94-100.

DOI：10.1007/s11427-010-0003-x

• GC-rich
• GC content
• DNA structure
• stable expression
• mammalian cell
• expression mechanism

High mammalian gene expression was obtained for more than twenty different proteins in different cell types by just a few laboratory scale stable gene transfections for each protein. The stable expression vectors were constructed by inserting a naturally-occurring 1.006 kb or a synthetic 0.733 kb DNA fragment (including intron) of extremely GC-rich at the 5' or/and 3' flanking regions of these protein genes or their gene promoters. This experiment is the first experimental evidence showing that a non-coding extremely GC-rich DNA fragment is a super "chromatin opening element" and plays an important role in mammalian gene expression. This experiment has further indicated that chromatin-based regulation of mammalian gene expression is at least partially embedded in DNA primary structure, namely DNA GC-content.

• 出版日期2010-1
• 单位中国人民解放军军事医学科学院