Phloroglucinols Inhibit Chemical Mediators and Xanthine Oxidase, and Protect Cisplatin-Induced Cell Death by Reducing Reactive Oxygen Species in Normal Human Urothelial and Bladder Cancer Cells

作者：Lin Kai Wei; Huang A Mei; Tu Huang Yao; Weng Jing Ru; Hour Tzyh Chyuan; Wei Bai Luh; Yang Shyh Chyun^*; Wang Jih Pyang; Pu Yeong Shiau; Lin Chun Nan

来源：Journal of Agricultural and Food Chemistry, 2009, 57(19): 8782-8787.

DOI：10.1021/jf900935n

• Phloroglucinol
• garcinielliptones HA-HE and anti-inflammatory
• antioxidant
• NTUB1
• SV-HUC1

Phloroglucinols, garcinielliptones HA-HE (1-5), and C (6) were studied in vitro for their inhibitory effects on chemical mediators released from mast cells, neutrophils, and macrophages. Compound 6 revealed significant inhibitory effect on release of lysozyme from rat neutrophils stimulated with formyl-Met-Leu-Phe (fMLP)/cytochalasin B (CB). Compounds 3, 4, and 6 showed significant inhibitory effects on superoxide anion generation in rat neutrophils stimulated with (fMLP)/(CB), while compounds 1 and 5 revealed inhibitory effects on tumor necrosis factor-alpha (TNF-alpha) formation in macrophages stimulated with lipopolysaccharide (LPS). Compounds 1 and 3-6 showed inhibitory effects on xanthine oxidase (XO) and could inhibit the DNA breakage caused by O-2(-center dot). Treatment of NTUB1 with 2 to 60 mu M compound 3 and 5 mu M cisplatin and SV-HUC1 with 9 to 60 mu M 3 and 5 mu M cisplatin, respectively, resulted in an increase of viability of cells. These results indicated that compounds 1 and 3-6 showed anti-inflammatory effects and antioxidant activities. Compound 3 mediates through the suppression of XO activity and reduction of reactive oxygen species (ROS), and protection of subsequent cell death.

• 出版日期2009-10-14