Actinomycin D binds to single stranded DNA oligomers which contain double GTC triplets

作者：Du, Weihong; Wang, Ling; Li, Juan; Wang, Baohuai; Li, Zhifen; Fang, Weihai^*

来源：Thermochimica Acta, 2007, 452(1): 31-35.

DOI：10.1016/j.tca.2006.10.011

• actinomycin D
• single stranded DNA
• GTC triplets
• thermodynamics

The present work carried out a study on the interactions between Actinomycin D (ActD) and some single-stranded DNA oligomers, which contain double GTC triplets separated by TTT sequence. The interactions of drugs with DNA oligomers were investigated by UV, circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC). The results indicate that ActD binds to the single stranded DNA oligomers in the fold back binding model as supported by added A/T base at DNA strand terminal which facilitates the formation of hairpin. The apparent binding constant K-b, the apparent binding molar enthalpy Delta H-0 and other thermodynamic data were obtained. The binding affinities are sequence dependent and related to the length of DNA strand. And the higher molar binding enthalpy indicates that the binding process is enthalpy driven.

• 出版日期2007-1-1
• 单位北京大学; 中国人民大学; 北京师范大学